The Human Leukemia Inhibitory Factor (LIF) ELISA Kit is specifically designed for the accurate measurement of LIF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.LIF is a key cytokine known for its role in regulating stem cell differentiation, immune response modulation, and embryo development. Dysregulation of LIF has been linked to various diseases, including leukemia, inflammatory disorders, and infertility. Therefore, the Human LIF ELISA Kit serves as an indispensable tool for investigating the pathophysiology of these conditions and exploring potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Human LIF ELISA Kit from Assay Genie is a valuable asset for researchers and clinicians seeking to advance their understanding of LIF biology and its implications in human health and disease.
Product Name:
Human LIF (Leukemia Inhibitory Factor) ELISA Kit
SKU:
HUES01380
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
