Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
The Human LP-PLA2 (Phospholipase A2) ELISA Kit is specifically designed for the accurate measurement of LP-PLA2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results, which makes it an excellent choice for various research applications.LP-PLA2, also known as lipoprotein-associated phospholipase A2, is an enzyme that plays a crucial role in the inflammation and development of atherosclerosis. Elevated levels of LP-PLA2 have been linked to an increased risk of cardiovascular events, making it a valuable biomarker for assessing cardiovascular disease risk and monitoring treatment effectiveness. By utilizing the Human LP-PLA2 ELISA Kit, researchers can gain valuable insights into the role of LP-PLA2 in various health conditions, including heart disease and stroke, and potentially identify new therapeutic targets for intervention. Overall, this kit provides a reliable tool for advancing our understanding of LP-PLA2 biology and its implications for human health.
Product Name:
Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
SKU:
HUES03168
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
