Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit
The Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit is specifically designed for the precise quantification of MCSF levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for a wide range of research applications.MCSF is a key signaling protein that plays a critical role in the regulation of macrophage function and differentiation. It is involved in various biological processes, including immune responses, inflammation, and tissue repair. Abnormal MCSF levels have been linked to diseases such as cancer, autoimmune disorders, and inflammatory conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human MCSF ELISA Kit offers researchers a reliable and efficient method for studying the role of MCSF in various physiological and pathological processes, facilitating advancements in biomedical research and drug discovery.
Product Name:
Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit
SKU:
HUES01383
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.