Human MHCG (Major Histocompatibility Complex Class I G) ELISA Kit
The Human MHC-I (Major Histocompatibility Complex Class I) ELISA Kit is specifically designed for the precise measurement of MHC-I levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The Major Histocompatibility Complex Class I proteins are essential for the immune system, playing a critical role in presenting antigens to T cells and initiating immune responses against pathogens. Dysregulation of MHC-I expression is implicated in various diseases, including autoimmune disorders and viral infections, making it a valuable biomarker for understanding these conditions and developing targeted therapies.With its high-quality components and user-friendly protocol, the Human MHC-I ELISA Kit from Assay Genie is a valuable tool for researchers studying immune responses, disease mechanisms, and potential treatment options. Trust in Assay Genie to provide reliable and accurate results for your MHC-I research needs.
Product Name:
Human MHCG (Major Histocompatibility Complex Class I G) ELISA Kit
SKU:
HUES02663
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
