Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit
The Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit is a powerful tool for the precise measurement of MIP-5 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.MIP-5, also known as CCL15, is a chemokine that plays a key role in immune responses and inflammation. It is involved in recruiting and activating immune cells, particularly monocytes and macrophages, to sites of inflammation. With its crucial role in inflammatory processes, MIP-5 serves as a valuable biomarker for studying inflammatory conditions and potentially developing therapeutic interventions.By utilizing the Human MIP-5 ELISA Kit, researchers can gain valuable insights into the role of MIP-5 in various diseases, such as autoimmune disorders, infectious diseases, and inflammatory conditions. With its high sensitivity and reliability, this kit is an essential tool for advancing research in the field of immunology and inflammation.
Product Name:
Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit
SKU:
HUES02547
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
