The Human MUC2 (Mucin 2) ELISA Kit is specifically designed for the accurate measurement of Mucin 2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Mucin 2 is a key component of the mucus layer that lines the gastrointestinal tract, playing a critical role in protecting the mucosal barrier and maintaining gut health. Dysregulation of Mucin 2 expression has been associated with various gastrointestinal disorders, including inflammatory bowel disease, colorectal cancer, and mucosal infections. As such, Mucin 2 serves as an important biomarker for understanding these conditions and exploring potential therapeutic interventions.Overall, the Human MUC2 ELISA Kit provides researchers with a valuable tool for investigating the role of Mucin 2 in gastrointestinal health and disease, offering insights into potential diagnostic and treatment strategies.
Product Name:
Human MUC2 (Mucin 2) ELISA Kit
SKU:
HUES01795
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
