Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
The Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit is thoughtfully engineered for the precise and quantitative detection of Neutrophil Gelatinase Associated Lipocalin in diverse human biological samples. NGAL is a crucial biomarker involved in various pathological conditions, particularly in acute kidney injury and chronic kidney disease, making it indispensable for monitoring renal function and identifying kidney-related issues at an early stage. Our ELISA kit is distinguished by its remarkable sensitivity and specificity, which guarantee accurate and reproducible results critical for renal health research. With a focus on stringent quality control standards, this kit delivers robust and consistent performance and is designed to be user-friendly, ensuring an excellent choice for your research needs. Trust in Assay Genie's NGAL ELISA Kit for reliable and precise quantification of this pivotal biomarker in your scientific investigations.
Product Name:
Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
SKU:
AEES00155
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
