Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit
The Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit is a powerful tool for the precise detection of NOX5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.NOX5 is a key enzyme involved in the production of reactive oxygen species, playing a crucial role in various physiological and pathological processes. Its dysregulation has been linked to conditions such as cancer, cardiovascular diseases, and inflammatory disorders, highlighting its importance as a valuable biomarker for research and therapeutic development.With its reliable performance and ease of use, the Human NOX5 ELISA Kit is an indispensable resource for researchers studying the role of NOX5 in health and disease, providing valuable insights into potential treatment strategies and disease mechanisms.
Product Name:
Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit
SKU:
HUES03115
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
