The Human NPY (Neuropeptide Y) ELISA Kit is specifically designed for the precise measurement of neuropeptide Y levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it a valuable tool for various research applications.Neuropeptide Y is a neurotransmitter that plays a crucial role in regulating appetite, stress response, and cardiovascular functions. Dysregulation of neuropeptide Y has been linked to various diseases such as obesity, anxiety disorders, and hypertension, making it a key biomarker for studying these conditions and developing potential therapies.Overall, the Human NPY (Neuropeptide Y) ELISA Kit provides researchers with a robust and accurate means of quantifying neuropeptide Y levels, aiding in the advancement of research in the field of neuroscience and related disciplines.
Product Name:
Human NPY (Neuropeptide Y) ELISA Kit
SKU:
HUES02841
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
