The Human Osteonectin ELISA Kit (HUES02397) is an advanced and reliable tool for the detection of osteonectin levels in human samples such as serum, plasma, and cell culture supernatants. Osteonectin, also known as SPARC (Secreted Protein Acidic and Rich in Cysteine), is a key extracellular matrix protein involved in bone development, wound healing, and tissue remodeling.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications. Osteonectin is a critical factor in various pathological conditions, including osteoporosis, cancer metastasis, and fibrosis, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions. With easy-to-follow protocols and rapid assay procedures, the Human Osteonectin ELISA Kit provides researchers with a powerful tool to investigate the role of osteonectin in health and disease. Order now to accelerate your research and gain valuable insights into the biology of osteonectin.
Product Name:
Human ON (Osteonectin) ELISA Kit
SKU:
HUES02397
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
