The Human PCI (Procollagen I) ELISA Kit is specifically designed for the precise measurement of procollagen I levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Procollagen I is a key protein involved in the synthesis and formation of collagen, the main structural protein in the human body. As collagen is essential for maintaining the structural integrity of tissues and organs, abnormalities in its production can lead to various health conditions, such as bone disorders, skin aging, and connective tissue diseases. Monitoring procollagen I levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With the Human PCI ELISA Kit, researchers can gain a deeper understanding of collagen synthesis and its impact on health and disease. This kit is a valuable tool for studying collagen-related disorders and exploring new treatment strategies.
Product Name:
Human PCI (Procollagen I) ELISA Kit
SKU:
HUES01448
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
