Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
The Assay Genie Human PCSK9 ELISA Kit is meticulously crafted for the precise and quantitative determination of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) levels in a variety of biological samples. PCSK9 is a critical enzyme known for its role in regulating low-density lipoprotein receptor (LDL-R) levels, thereby impacting cholesterol metabolism and cardiovascular health. Accurate measurement of PCSK9 is essential in understanding its involvement in lipid metabolism, cardiovascular diseases, and potential therapeutic interventions. Our PCSK9 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research endeavors. Manufactured under strict quality control standards, this kit delivers robust performance and ease of use, making it a top choice for investigations in lipid biology, cardiovascular research, and related fields. Trust Assay Genie's PCSK9 ELISA Kit for reliable and high-quality quantification of PCSK9, empowering your scientific studies with precise data and insights.
Product Name:
Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
SKU:
AEES00182
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
