Human PDGF-AA (Platelet Derived Growth Factor AA) ELISA Kit
The Assay Genie Human PDGF-AA ELISA Kit is meticulously designed for the sensitive and quantitative measurement of Platelet-Derived Growth Factor AA (PDGF-AA) levels in various biological samples. PDGF-AA, a crucial cell growth factor, plays a significant role in cell proliferation, wound healing, angiogenesis, and tissue repair processes. Accurate quantification of PDGF-AA is essential for understanding its role in various cellular functions and its implications in conditions related to growth regulation and tissue repair. Our PDGF-AA ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research endeavors. Manufactured under stringent quality control standards, this kit guarantees robust performance and ease of use, making it an ideal choice for studies focusing on cell growth, angiogenesis, and related mechanisms. Trust Assay Genie's PDGF-AA ELISA Kit for precise and dependable quantification of PDGF-AA, empowering your scientific exploration and discovery in the field of growth factor biology.
Product Name:
Human PDGF-AA (Platelet Derived Growth Factor AA) ELISA Kit
SKU:
AEES00183
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
