Human Phospho-Estrogen receptor (S118) PharmaGenie ELISA Kit (SBRS1813)

SKU:
SBRS1813
Product type:
ELISA
Reactivity:
Human
Application:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-Estrogen receptor S118 PharmaGenie ELISA Kit SBRS1813
  • Human Phospho-Estrogen receptor S118 PharmaGenie ELISA Kit SBRS1813
€713

Description

Human Phospho-Estrogen receptor (S118) PharmaGenie ELISA - Kit Data

Product SKU:SBRS1813
Size:96T
Application:The antibody pair provided in this kit recognizes Human Estrogen receptor phosphorylated at site Serine-118.
Uniprot:P03372
Gene ID:2099
Gene Names:ESR1
Pathway:MAPK Signaling
Synonyms:Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.

Human Phospho-Estrogen receptor (S118) PharmaGenie ELISA Kit (SBES1813) - Product Images

Product ImageJurkat cells were treated or untreated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA.
Product ImageZR751 cells were treated with b-estradiol at 37°C for 1 hours. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Anti-Phospho Antibody
4.HRP-Conjugated Secondary Antibody
5.Assay Diluent
6.TMB One-Step Substrate
7.Stop Solution
8.Lysis Buffer
9.Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-Estrogen receptor (S118) ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.
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