Human PIICP (Procollagen II C-Terminal ProPeptide) ELISA Kit
The Human PIICP (Procollagen II C-terminal Propeptide) ELISA Kit is a powerful tool for the precise measurement of PIICP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PIICP is a vital component in collagen synthesis and is specifically involved in the production of type II collagen, which is essential for maintaining healthy cartilage and connective tissues. Dysregulation of PIICP levels has been linked to conditions such as osteoarthritis, rheumatoid arthritis, and other musculoskeletal disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human PIICP ELISA Kit provides researchers with a reliable method for investigating PIICP levels in human samples, offering insights into the pathophysiology of various diseases and opening avenues for novel treatment strategies.
Product Name:
Human PIICP (Procollagen II C-Terminal ProPeptide) ELISA Kit
SKU:
HUES02102
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
