Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
The Human pMAPT (pTau, Phosphorylated Microtubule-Associated Protein Tau) ELISA Kit is specifically designed for the accurate measurement of phosphorylated microtubule-associated protein tau levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it suitable for various research purposes.Phosphorylated microtubule-associated protein tau, or pTau, plays a key role in neurodegenerative disorders such as Alzheimer's disease, where abnormal tau phosphorylation leads to the formation of neurofibrillary tangles. By measuring pTau levels, researchers can gain valuable insights into the progression of these disorders and potentially identify new therapeutic targets.This ELISA kit is a valuable tool for studying the pathophysiology of neurodegenerative diseases and exploring potential treatment options. Its ease of use and efficiency make it an essential component for any laboratory conducting research in this area.
Product Name:
Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
SKU:
HUES03407
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
