The Human PON1 (Paraoxonase 1) ELISA Kit is specifically designed for the accurate measurement of paraoxonase 1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Paraoxonase 1 is an important enzyme that plays a key role in protecting against oxidative stress and lipid peroxidation. It is involved in the metabolism of organophosphate compounds and is known to have anti-inflammatory and antioxidant properties. Studies have shown that paraoxonase 1 levels may be altered in various diseases such as cardiovascular diseases, diabetes, and Alzheimer's disease, making it a valuable biomarker for researchers studying these conditions and exploring potential therapeutic interventions.With its superior performance and user-friendly design, the Human PON1 ELISA Kit from Assay Genie is a valuable tool for researchers looking to accurately measure paraoxonase 1 levels in human samples and advance their understanding of its role in health and disease.
Product Name:
Human PON1 (Paraoxonase 1) ELISA Kit
SKU:
HUES03175
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
