Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
The Human PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) ELISA Kit is specifically designed for the accurate and reliable detection of PPAR Gamma levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for a variety of research applications.PPAR Gamma is a key transcription factor involved in the regulation of numerous biological processes, including lipid metabolism, insulin sensitivity, and inflammatory responses. Dysregulation of PPAR Gamma has been linked to various diseases such as obesity, diabetes, and cardiovascular disorders, making it a vital target for drug development and therapeutic interventions.By utilizing the Human PPAR Gamma ELISA Kit, researchers can gain valuable insights into the role of PPAR Gamma in various physiological and pathological conditions, ultimately advancing our understanding of disease mechanisms and potential treatment options. Elevate your research with this innovative and reliable ELISA kit from Assay Genie.
Product Name:
Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
SKU:
HUES02406
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
