Human ProGRP (Pro-Gastrin Releasing Peptide) ELISA Kit
The Human ProGRP (Pro Gastrin Releasing Peptide) ELISA Kit is specifically designed for the precise measurement of ProGRP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.ProGRP is a vital biomarker associated with various cancers, particularly small cell lung cancer and neuroendocrine tumors. Its detection can aid in early diagnosis, prognosis, and monitoring of these diseases, making it a valuable tool in research and clinical settings. With reliable performance and easy-to-use protocols, the Human ProGRP ELISA Kit from AssayGenie is an essential tool for studying the role of ProGRP in cancer biology and developing potential diagnostic and therapeutic strategies. Suitable for both basic research and clinical applications, this kit is a valuable asset for any laboratory focused on cancer research and biomarker discovery.
Product Name:
Human ProGRP (Pro-Gastrin Releasing Peptide) ELISA Kit
SKU:
HUES02976
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.