The Human RETN (Resistin) ELISA Kit is specifically designed for the precise measurement of resistin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Resistin is a key adipokine that plays a critical role in regulating insulin sensitivity, inflammation, and metabolism. Elevated resistin levels have been linked to various metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. As such, this ELISA kit is an invaluable tool for investigating the role of resistin in these conditions and exploring potential therapeutic interventions.Overall, the Human RETN (Resistin) ELISA Kit provides researchers with a reliable and efficient method for quantifying resistin levels in various biological samples, facilitating in-depth studies on its physiological and pathological functions.
Product Name:
Human RETN (Resistin) ELISA Kit
SKU:
HUES02284
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
