Human S100A9 (S100 Calcium Binding Protein A9) CLIA Kit
The Human S100A9 (S100 Calcium Binding Protein A9) CLIA Kit is specifically designed for the precise and reliable detection of S100A9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.S100A9, also known as S100A9, is a calcium-binding protein that plays a crucial role in inflammation, immune response, and cell differentiation.
Elevated levels of S100A9 have been linked to various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human S100A9 CLIA Kit is an essential tool for researchers looking to investigate the role of S100A9 in various diseases and for developing novel diagnostic and treatment strategies.
Product Name:
Human S100A9 (S100 Calcium Binding Protein A9) CLIA Kit
SKU:
HUES00742
Target:
Human S100A9 (S100 Calcium Binding Protein A9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A9. You can calculate the concentration of Human S100A9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
103-120
93-105
Average (%)
90
109
99
1:4
Range (%)
102-121
98-114
95-109
Average (%)
110
104
102
1:8
Range (%)
94-106
96-111
96-112
Average (%)
100
102
104
1:16
Range (%)
98-111
96-111
99-117
Average (%)
103
101
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
91-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.49
4.04
0.53
1.43
4.14
Standard deviation
0.05
0.13
0.41
0.06
0.17
0.34
C V (%)
9.43
8.72
10.15
11.32
11.89
8.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A9 in samples. No significant cross-reactivity or interference between Human S100A9 and analogues was observed.
