Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
The Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit is meticulously designed for the precise and quantitative assessment of sCD14 levels in a variety of human biological samples. sCD14, a crucial protein known for its role in immune responses and inflammatory processes, serves as a key player in modulating immune reactions, specifically in sensing pathogens and activating immune cells. Accurate quantification of sCD14 levels is vital for unraveling its contributions to immune-related disorders, inflammatory conditions, and infectious diseases. Our ELISA kit offers unparalleled sensitivity and specificity, ensuring the generation of reliable and reproducible data essential for exploring the diverse functions of sCD14. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an optimal solution for cutting-edge research endeavors. Choose Assay Genie's sCD14 ELISA Kit for dependable and precise quantification of this significant biomarker in your research studies.
Product Name:
Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
SKU:
AEES00158
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
