The Assay Genie Human SELP (P-Selectin) ELISA Kit is expertly designed to facilitate the precise quantitative measurement of P-Selectin levels in a variety of biological samples. P-Selectin, also known as SELP, is a crucial adhesion molecule that plays a fundamental role in mediating leukocyte rolling and promoting interactions between leukocytes and endothelial cells during the inflammatory response. By accurately quantifying P-Selectin levels, researchers can gain valuable insights into the mechanisms underlying inflammation, immune responses, and various inflammatory disorders. Our SELP ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your investigations. Manufactured under stringent quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research applications. Trust Assay Genie's SELP ELISA Kit for dependable and precise quantification of P-Selectin, empowering your studies in immunology and beyond.
Product Name:
Human SELP (P-Selectin) ELISA Kit
SKU:
AEES00180
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
