Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
The Human SPA (Pulmonary Surfactant-associated Protein A) ELISA Kit is a reliable and accurate tool for detecting levels of SPA in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.SPA is a key protein involved in pulmonary surfactant function, essential for maintaining normal lung function and preventing respiratory distress syndrome. Dysregulation of SPA levels has been associated with various pulmonary diseases, making it a valuable biomarker for studying and developing therapies for respiratory conditions.With its advanced technology and proven performance, the Human SPA ELISA Kit is a valuable asset for researchers studying lung biology, respiratory diseases, and therapeutic interventions. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
SKU:
HUES02324
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
