Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor) ELISA Kit
The suPAR ELISA Kit is designed for the quantitative detection of Soluble Urokinase-type Plasminogen Activator Receptor (suPAR) levels in various human biological samples. suPAR is a critical biomarker involved in the regulation of immune and inflammatory responses, including cell adhesion, migration, and tissue remodeling. Its measurement can provide insights into various pathological states including infection, inflammation, and malignancy, making it an important target for disease monitoring and therapeutic intervention. Assay Genie's suPAR ELISA Kit offers superior sensitivity and specificity, ensuring reliable and reproducible results. Produced under strict quality control standards, this kit provides strong performance and is user-friendly, making it an excellent choice for both research and clinical applications. Trust Assay Genie's suPAR ELISA Kit for accurate and dependable quantification of this important biomarker in your studies.
Product Name:
Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor) ELISA Kit
SKU:
AEES00035
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.