The Human SYNPO (Synaptopodin) ELISA Kit is specifically designed for the precise measurement of synaptopodin levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Synaptopodin is a key protein involved in the regulation of actin dynamics in dendritic spines, contributing to synaptic plasticity and neuronal function. Dysregulation of synaptopodin has been implicated in various neurological disorders, including Alzheimer's disease and schizophrenia, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.Overall, the Human SYNPO (Synaptopodin) ELISA Kit provides researchers with a valuable tool for investigating the role of synaptopodin in neuronal function and its potential implications in neurodegenerative diseases.
Product Name:
Human SYNPO (Synaptopodin) ELISA Kit
SKU:
HUES02943
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
