Human TACE (TNF alpha Converting Enzyme) ELISA Kit
The Human TACE (TNF-alpha Converting Enzyme) ELISA Kit is specially designed for the precise and sensitive detection of TACE levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high accuracy and reliability, ensuring consistent and reproducible results, making it perfect for a variety of research purposes.TACE, also known as ADAM17, is a key enzyme involved in the shedding of cell surface molecules, including pro-inflammatory cytokines like TNF-alpha. Dysregulation of TACE activity has been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.This ELISA kit provides researchers with a powerful tool to study the role of TACE in inflammatory pathways, paving the way for new insights and discoveries in the field of immunology and inflammation-related diseases. Get your Human TACE ELISA kit now and unlock the potential of your research.
Product Name:
Human TACE (TNF alpha Converting Enzyme) ELISA Kit
SKU:
HUES03182
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
