Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit
The Human TFF3 (Trefoil Factor 3) Intestinal ELISA Kit is specifically designed for the quantitative detection of TFF3 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and accurate, ensuring precise and consistent results for a variety of research applications.TFF3, also known as trefoil factor 3, is a key protein involved in mucosal protection and repair in the gastrointestinal tract. It plays a crucial role in maintaining the integrity of the intestinal lining and promoting healing of damaged tissue. Dysregulation of TFF3 levels has been associated with digestive disorders such as inflammatory bowel disease (IBD) and gastric ulcers, making it a valuable biomarker for studying these conditions and developing potential treatments. With its advanced technology and proven performance, the Human TFF3 Intestinal ELISA Kit from Assay Genie is a valuable tool for researchers studying the role of TFF3 in gastrointestinal health and disease. Order yours today to advance your research and make impactful discoveries in the field of gastrointestinal biology.
Product Name:
Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit
SKU:
HUES02200
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
