Human TJP1 (Tight junction protein ZO-1) ELISA Kit
The Human TJP1 (Tight Junction Protein ZO-1) ELISA Kit is specifically designed for the accurate measurement of TJP1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TJP1, also known as ZO-1, is a key protein involved in maintaining the integrity of tight junctions between cells. These tight junctions play a critical role in regulating the passage of molecules and ions across cell barriers, making TJP1 essential for proper cell function. Dysregulation of TJP1 has been implicated in various diseases, including inflammatory disorders, autoimmune diseases, and cancer, highlighting its significance as a potential biomarker for studying and developing therapies for these conditions.Overall, the Human TJP1 ELISA Kit provides researchers with a reliable tool for studying the role of TJP1 in various physiological and pathological processes, ultimately contributing to a better understanding of tight junction biology and its implications for human health.
Product Name:
Human TJP1 (Tight junction protein ZO-1) ELISA Kit
SKU:
HUES02538
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
