The TSH ELISA Kit is designed for the quantitative detection of Thyroid Stimulating Hormone (TSH) levels in various human biological samples. TSH is a critically important hormone involved in the regulation of metabolic processes, integral in the stimulation of thyroid hormone production and release. It plays a significant role in the body's metabolic activities and disturbances in its levels can lead to various conditions like hyperthyroidism or hypothyroidism. Accurate measurement of TSH is imperative for understanding its role in metabolic regulation and for the development of effective therapeutic strategies. Assay Genie's TSH ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's TSH ELISA Kit for accurate and reliable quantification of this crucial biomarker in your studies.
Product Name:
Human TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
AEES00037
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 μIU/mL
Detection range:
0.31-20 μIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
