Human TSLP (Thymic Stromal Lymphopoietin) ELISA Kit
The Human TSLP (Thymic Stromal Lymphopoietin) ELISA Kit is specifically designed for the accurate detection of TSLP levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TSLP is a key protein involved in immune responses and inflammatory processes, playing a critical role in conditions such as asthma, dermatitis, and other allergic diseases. By measuring TSLP levels, researchers can gain valuable insights into the pathogenesis of these conditions and develop targeted therapeutic interventions.With its advanced technology and reliable performance, the Human TSLP ELISA Kit from AssayGenie is an essential tool for studying the role of TSLP in disease progression and developing new treatment strategies.
Product Name:
Human TSLP (Thymic Stromal Lymphopoietin) ELISA Kit
SKU:
HUES02607
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.