Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
The TWEAK ELISA Kit is designed for the quantitative detection of Tumour Necrosis Factor-Related Weak Inducer of Apoptosis (TWEAK) levels in various human biological samples. TWEAK is a multifunctional cytokine involved in diverse cellular processes, including cell death, proliferation, and differentiation. It plays a significant role in regulating inflammation and apoptosis and has implications in various disease conditions and cellular responses. Accurate measurement of TWEAK is crucial for understanding its functions and its potential role in disease pathology and therapeutic strategies. Our TWEAK ELISA Kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols suitable for both research and clinical applications. Trust our TWEAK ELISA Kit for precise and reliable quantification of this essential biomarker in your studies and investigations.
Product Name:
Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
SKU:
AEES00041
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
