Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
The Human UCP-1 (Uncoupling Protein-1) Mitochondrial ELISA Kit is a specialized assay designed for the precise measurement of UCP-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.UCP-1 is a pivotal protein involved in metabolic processes, particularly in the regulation of energy expenditure and thermogenesis. Its role in brown adipose tissue activation has garnered significant interest in the study of obesity, diabetes, and metabolic disorders. Understanding UCP-1 levels can provide valuable insights into these conditions and aid in the development of therapeutic interventions.Whether investigating the mechanisms of UCP-1 function or exploring its potential as a biomarker for metabolic health, the Human UCP-1 Mitochondrial ELISA Kit is a valuable tool for researchers seeking to uncover the complexities of energy metabolism and metabolic diseases.
Product Name:
Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
SKU:
HUES02661
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
