Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
The Assay Genie Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit is meticulously crafted to facilitate the quantitative detection of VEGFR1/FLT1 levels in diverse biological samples. VEGFR1, as a pivotal player in angiogenesis, vascular development, and endothelial cell function, is crucial for regulating critical processes like vascular permeability and cell survival. By accurately quantifying VEGFR1/FLT1 levels, researchers can garner valuable insights into angiogenic signaling pathways and vascular-related pathologies.
Our VEGFR1/FLT1 ELISA Kit boasts outstanding sensitivity and specificity, ensuring the generation of reliable and reproducible data for your research needs. Manufactured under stringent quality control standards, this kit promises robust performance and ease of use, making it an optimal solution for conducting in-depth investigations in the field of VEGFR1-related research. Trust Assay Genie's VEGFR1/FLT1 ELISA Kit for precise and dependable quantification, empowering your scientific explorations.
Product Name:
Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
SKU:
AEES00175
Target:
Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR1/FLT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR1/FLT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR1/FLT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR1/FLT1. You can calculate the concentration of Human VEGFR1/FLT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
86-99
99-112
Average (%)
106
91
104
1:4
Range (%)
98-110
82-96
95-107
Average (%)
104
88
100
1:8
Range (%)
97-112
85-98
93-108
Average (%)
104
92
100
1:16
Range (%)
97-110
83-97
93-109
Average (%)
103
89
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.21
203.54
957.45
109.81
222.5
908.1
Standard deviation
6.06
10.42
42.99
5.98
11.75
44.22
C V (%)
5.65
5.12
4.49
5.45
5.28
4.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGFR1/FLT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGFR1/FLT1 in samples. No significant cross-reactivity or interference between Human VEGFR1/FLT1 and analogues was observed.
