The Human VIM (Vimentin) ELISA Kit is a powerful tool for accurately detecting vimentin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results that are suitable for a variety of research applications.Vimentin is an important protein involved in maintaining cellular structure and integrity, playing a key role in cell migration and signaling pathways.
Abnormal vimentin expression is associated with various diseases including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for disease progression and therapeutic interventions.With its reliable performance and versatility, the Human VIM (Vimentin) ELISA Kit is an essential tool for researchers studying vimentin biology and its implications in various health conditions.
Product Name:
Human VIM (Vimentin) ELISA Kit
SKU:
HUES02188
Target:
Human VIM (Vimentin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VIM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VIM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VIM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VIM. You can calculate the concentration of Human VIM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
87-99
94-106
Average (%)
92
94
100
1:4
Range (%)
89-103
82-93
83-94
Average (%)
96
87
88
1:8
Range (%)
88-101
79-89
87-99
Average (%)
93
85
94
1:16
Range (%)
94-108
83-96
83-93
Average (%)
100
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.1
76.86
213.21
24.59
72.07
200.14
Standard deviation
1.34
4.53
8.38
1.47
4.12
7.55
C V (%)
5.56
5.89
3.93
5.98
5.72
3.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VIM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed.
