The Human VWF (Von Willebrand Factor) ELISA Kit is a highly reliable and accurate tool for the quantitative detection of VWF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Von Willebrand Factor is a crucial protein involved in blood clotting and platelet adhesion, playing a key role in maintaining vascular integrity and hemostasis. Dysregulation of VWF levels is associated with various bleeding disorders and thrombotic diseases, highlighting its importance as a biomarker for studying these conditions and developing targeted therapies.Overall, the Human VWF ELISA Kit is an essential tool for researchers investigating the role of VWF in health and disease, providing valuable insights into the mechanisms underlying hemostasis and thrombosis.
Product Name:
Human VWF (Von Willebrand Factor) ELISA Kit
SKU:
HUES03069
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
