Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit
The Human Wingless-type MMTV Integration Site Family Member 3A (WNT3A) ELISA Kit is specifically designed for the precise and sensitive detection of WNT3A levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, allowing for accurate analysis in a variety of research settings.WNT3A is a key member of the WNT signaling pathway, playing a critical role in cell proliferation, differentiation, and migration.
Dysregulation of WNT3A has been linked to various diseases such as cancer, osteoporosis, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its high sensitivity and specificity, the Human WNT3A ELISA Kit is an essential tool for researchers looking to investigate the role of WNT3A in disease pathogenesis and explore potential therapeutic targets.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit
Product Code:
HUDL03128
Size:
96 Assays
Target:
WNT3A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
