The IFN gamma Monoclonal Antibody (CAB23929) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. RRID Gene ID 3458 Swiss Prot Synonym IFG; IFI; IMD69; IFN gamma
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IFN gamma Monoclonal Antibody
SKU:
CAB23929
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC52882
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IFG, IFI, IMD69, IFN gamma
Positive Sample:
Recombinant Human IFN gamma Protein
Cellular Localization:
Secreted.
Calculated MW:
19kDa
Observed MW:
17kDa
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. RRID Gene ID 3458 Swiss Prot Synonym IFG; IFI; IMD69; IFN gamma
Purification Method:
Affinity purification
Gene ID:
3458
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Human IFN-gamma Protein (RP01038), using IFN gamma Rabbit mAb (CAB23929) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
