The ILKAP Antibody (CAB14305) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to Ilkap, researchers can detect and analyze the protein in various cell types, making it an essential asset for studies in molecular biology and cell biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ILKAP Antibody
SKU:
CAB14305
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP2CD, PPM1O, ILKAP2, ILKAP3, PP2C-DELTA, ILKAP
Positive Sample:
HeLa, HepG2, Mouse spleen, Mouse testis
Cellular Localization:
Cytoplasm.
Calculated MW:
43kDa
Observed MW:
42kDa
The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.
Purification Method
Affinity purification
Gene ID
80895
RRID
AB_2761168
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ILKAP Rabbit pAb (CAB14305) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
