The IP3 (Inositol Triphosphate) ELISA Kit is a reliable and efficient tool for the accurate measurement of IP3 levels in a variety of sample types including cell lysates, tissue homogenates, and biological fluids. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in a wide range of research applications.IP3 is a crucial second messenger molecule involved in signaling pathways that regulate various cellular processes including cell growth, differentiation, and metabolism. Dysregulation of IP3 levels has been implicated in numerous diseases such as cancer, neurological disorders, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the IP3 ELISA Kit is a valuable tool for researchers seeking to investigate the role of IP3 in cellular signaling pathways and its potential implications in disease pathogenesis.
Product Name:
IP3 (Inositol Triphosphate) ELISA Kit
SKU:
UNES00039
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
