Anti-GATA2/3 Antibody (CAB5083)

Antibody Name:	Anti-GATA2/3 [KO Validated] Antibody
Antibody SKU:	CAB5083
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human GATA2/3

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse lung, Rat lung

Immunogen:	A synthesized peptide derived from human GATA2/3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2624
Uniprot:	P23769/P23771
Cellular Location:	Nucleus
Calculated MW:	51kDa
Observed MW:	51KDa

Synonyms:

DCML, IMD21, MONOMAC, NFE1B

Background:

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

UniProt Protein Function:	GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: nucleoplasm; protein complex Molecular Function:protein binding; zinc ion binding; chromatin binding; transcription factor activity; transcription factor binding Biological Process: inner ear morphogenesis; embryonic placenta development; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of erythrocyte differentiation; cell maturation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; negative regulation of fat cell differentiation; phagocytosis; cell differentiation in hindbrain; positive regulation of angiogenesis; negative regulation of macrophage differentiation; homeostasis of number of cells within a tissue; commitment of a neuronal cell to a specific type of neuron in the forebrain; pituitary gland development; ventral spinal cord interneuron differentiation; positive regulation of phagocytosis; negative regulation of Notch signaling pathway; positive regulation of megakaryocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; urogenital system development; central nervous system neuron development Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome
NCBI Summary:	This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
UniProt Code:	P23769
NCBI GenInfo Identifier:	229462971
NCBI Gene ID:	2624
NCBI Accession:	P23769.3
UniProt Secondary Accession:	P23769,Q53YE0, Q96BH0, Q96BH8, Q9BUJ6, D3DNB3,
UniProt Related Accession:	P23769
Molecular Weight:	480
NCBI Full Name:	Endothelial transcription factor GATA-2
NCBI Synonym Full Names:	GATA binding protein 2
NCBI Official Symbol:	GATA2
NCBI Official Synonym Symbols:	DCML; IMD21; NFE1B; MONOMAC
NCBI Protein Information:	endothelial transcription factor GATA-2
UniProt Protein Name:	Endothelial transcription factor GATA-2
UniProt Synonym Protein Names:	GATA-binding protein 2
Protein Family:	GATA-binding factor
UniProt Gene Name:	GATA2
UniProt Entry Name:	GATA2_HUMAN

	Western blot analysis of extracts of various cell lines, using GATA2/3 Rabbit mAb (CAB5083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Western blot analysis of extracts from normal (control) and GATA2/3 knockout (KO) HeLa cells, using GATA2/3 antibody (CAB5083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)