The MDA (Malondialdehyde) ELISA kit offered by Assay Genie is designed for the accurate detection of malondialdehyde levels in various biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a range of research applications.Malondialdehyde is a marker for oxidative stress and lipid peroxidation, which are implicated in a variety of pathological conditions including cardiovascular diseases, neurodegenerative disorders, and inflammatory diseases. The measurement of malondialdehyde levels can provide valuable insights into the extent of oxidative damage and serve as a biomarker for monitoring disease progression and evaluating potential therapeutic interventions.With its advanced technology and reliable performance, the MDA ELISA kit from Assay Genie is an indispensable tool for researchers studying oxidative stress-related diseases and developing novel treatments to combat these conditions. Order your kit today and unlock new possibilities in your research endeavors.
Product Name:
MDA (Malondialdehyde) ELISA Kit
SKU:
UNES00040
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
