The Mouse IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) ELISA (Small Sample Volume) is designed for the quantitative detection of Mouse IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) levels in various biological samples. Interleukin-1 (IL-1) is a principal mediator of innate immunity, produced primarily by activated macrophages and monocytes in response to infection or tissue injury. It drives fever induction, acute-phase protein synthesis, leukocyte recruitment, and activation of adaptive immune responses through NF-κB and MAPK signaling. Dysregulation of IL-1 signaling is central to autoinflammatory syndromes, rheumatoid arthritis, gout, and sepsis-associated organ dysfunction. Assay Genie's Mouse IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 15.75 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Mouse IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
94-111
93-109
98-111
Average (%)
102
100
104
1:4
Range (%)
97-113
95-111
97-112
Average (%)
103
102
103
1:8
Range (%)
91-104
97-111
94-105
Average (%)
99
102
100
1:16
Range (%)
91-106
90-103
100-116
Average (%)
97
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
92-109
99
Cell Culture Media (n=8)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.61
178.19
811.97
95.62
182.86
747.5
Standard deviation
5.72
10.99
44.58
7.46
11.56
53.0
C V (%)
6.11
6.17
5.49
7.8
6.32
7.09
Uniport ID:
P25085
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 25 μL
Specificity:
This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed.
