The Mouse ANG II (Angiotensin II) ELISA Kit is specifically designed for the accurate detection of Angiotensin II levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Angiotensin II is a key peptide hormone involved in regulating blood pressure, fluid balance, and electrolyte homeostasis. It plays a crucial role in the renin-angiotensin system, impacting various physiological processes such as vasoconstriction, inflammation, and cell growth. Abnormal Angiotensin II levels have been linked to conditions such as hypertension, heart failure, and kidney disease, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions. With its reliable performance and ease of use, the Mouse ANG II ELISA Kit is an essential tool for researchers investigating the role of Angiotensin II in physiological and pathological processes. Get accurate and reproducible results with this comprehensive kit, advancing your understanding of Angiotensin II biology and its implications in disease development and treatment.
Product Name:
Mouse Ang-II (Angiotensin II) ELISA Kit
SKU:
MOES01753
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
