The Mouse ANP (Atrial Natriuretic Peptide) ELISA Kit is specifically designed for the accurate quantification of ANP levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers in various fields.ANP is a key peptide involved in regulating blood pressure and fluid balance in the body. It plays a critical role in cardiovascular health, making it a valuable biomarker for studying heart-related conditions and potential therapeutic interventions. With this ELISA kit, researchers can efficiently measure ANP levels and advance their understanding of cardiovascular physiology and pathology in mouse models.Overall, the Mouse ANP ELISA Kit from Assay Genie provides a reliable tool for researchers to conduct accurate and comprehensive studies on ANP biology and its implications in cardiovascular health.
Product Name:
Mouse ANP (Atrial Natriuretic Peptide) ELISA Kit
SKU:
MOES00752
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
