Mouse CXCL9/MIG (Monokine induced by interferon-gamma) CLIA Kit
The Mouse CXCL9 (MIG) CLIA Kit is a powerful tool for measuring monokine induced by interferon gamma (MIG) levels in mouse samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it invaluable for a variety of research applications.MIG is a key chemokine involved in immune responses, particularly in inflammation and the recruitment of immune cells to sites of infection.
Its dysregulation has been implicated in various autoimmune diseases and cancer, highlighting the importance of studying MIG levels in preclinical research and therapeutic development.With the Mouse CXCL9 (MIG) CLIA Kit, researchers can gain valuable insights into immune responses and potential therapeutic targets in mouse models, ultimately advancing our understanding of disease mechanisms and treatment strategies.
Product Name:
Mouse CXCL9/MIG (Monokine induced by interferon-gamma) CLIA Kit
SKU:
MOES00018
Target:
Mouse CXCL9/MIG (Monokine induced by interferon-gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL9/MIG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL9/MIG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL9/MIG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CXCL9/MIG. You can calculate the concentration of Mouse CXCL9/MIG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
99-109
85-98
Average (%)
107
104
92
1:4
Range (%)
89-101
93-108
100-118
Average (%)
94
101
108
1:8
Range (%)
97-112
100-113
87-102
Average (%)
104
107
94
1:16
Range (%)
86-100
95-111
91-103
Average (%)
92
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.54
142.04
469.71
52.58
129.98
462.13
Standard deviation
5.49
10.13
43.96
5.96
14.15
31.93
C V (%)
11.31
7.13
9.36
11.34
10.89
6.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CXCL9/MIG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCL9/MIG in samples. No significant cross-reactivity or interference between Mouse CXCL9/MIG and analogues was observed.
