The Mouse ELAV-like protein 1 (ELAVL1) ELISA Kit is a powerful tool for the accurate measurement of ELAVL1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring dependable and consistent results that are suitable for a variety of research applications.ELAVL1, also known as Hu protein R, is a RNA-binding protein that plays a critical role in regulating mRNA stability and translation. It is involved in a variety of biological processes, including neuronal development, synaptic plasticity, and stress response.
Dysregulation of ELAVL1 has been implicated in neurological disorders, cancer, and inflammatory diseases, highlighting its importance as a potential biomarker for disease research and therapeutic development.The Mouse ELAVL1 ELISA Kit from AssayGenie provides researchers with a reliable and accurate tool for quantifying ELAVL1 levels in biological samples, offering valuable insights into its function and potential impact on disease pathology.
Product Name:
Mouse ELAV Like Protein 1 (ELAVL1) ELISA Kit
Product Code:
MODL00417
Size:
96 Assays
Target:
ELAVL1
Synonyms:
ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
