The Mouse EPO (Erythropoietin) ELISA Kit is a sophisticated tool designed to quantitatively measure Erythropoietin levels in a variety of biological samples with accuracy and precision. Erythropoietin, a pivotal hormone primarily secreted by the kidneys, plays a crucial role in regulating red blood cell production, also known as erythropoiesis. Accurate quantification of Erythropoietin is essential for investigating its involvement in erythropoiesis, anemia, and hematological disorders. As a vital regulator of red blood cell production, understanding Erythropoietin levels provides valuable insights into hematopoiesis and erythrocyte function. Through the use of the Mouse EPO ELISA Kit, researchers can unlock crucial information regarding Erythropoietin's function in hematological processes and anemia, paving the way for the development of targeted therapeutic interventions. The Mouse EPO ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results. Manufactured under strict quality control guidelines, this ELISA kit delivers consistent and robust performance, making it an ideal choice for researchers aiming to explore the role of Erythropoietin in hematopoiesis and hematologic disorders.
Product Name:
Mouse EPO (Erythropoietin) ELISA Kit
SKU:
AEES00249
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
