The Mouse IL-11 ELISA Kit is specially designed for the precise measurement of interleukin-11 (IL-11) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.IL-11 is a key cytokine that plays a vital role in various physiological processes, including hematopoiesis, immune response, and bone metabolism. Dysregulation of IL-11 levels has been implicated in inflammatory diseases, cancer, and bone disorders, highlighting its importance as a biomarker for studying and developing therapeutic interventions for these conditions. With its advanced technology and optimized protocols, the Mouse IL-11 ELISA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of IL-11 in health and disease. Trust in its quality and performance to advance your research endeavors and uncover new insights into the intricate mechanisms involving IL-11 in mouse models.
Product Name:
Mouse IL-11 ELISA Kit
SKU:
MOES01220
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
