The Mouse IL-15 ELISA Kit is a powerful tool for detecting and quantifying levels of IL-15 in mouse serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.IL-15 is a key cytokine involved in the regulation of immune responses, playing a critical role in the activation and proliferation of T cells and natural killer cells. Dysregulation of IL-15 has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer, making it an important target for research and potential therapeutic interventions. With the Mouse IL-15 ELISA Kit, researchers can gain valuable insights into the mechanisms underlying immune responses and disease pathology, paving the way for novel treatments and diagnostic strategies. Trust in the reliability and precision of this kit to advance your research endeavors in immunology, oncology, and beyond.
Product Name:
Mouse IL-15 ELISA Kit
SKU:
MOES01223
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
